Figure 4.

Dose-response effect of pRBC transfusion on the plasma metabolome heat maps of top 25 metabolites that changed significantly in response to transfusion as measured by ANOVA (y-axis) with patients on the x-axis (A). The heat maps were divided into transfusion phases “pre-during-post” grouped by the number of transfusion “events” 1–5. In panel (B), PLS-DA of patient metabolomes grouped by transfusion phase. Patients that received strictly greater than one transfusion event were included in PLS-DA. In (C) we show box plots of select metabolites that changed significantly in response to transfusions. Box plot colors correspond to pre and post transfusion time points, consistent with panels (A,B). **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.