FIG. 1.

Production of cytokines by splenocytes from noninfected mice immunized with WR201 9 weeks previously (striped bars) or from noninfected, nonimmunized mice (stippled bars). Splenocytes were cultured for 24 h (IL-2 and IFN-γ) or 48 h (IL-10) with medium, 2 μg of ConA, heat-killed 16M (HK), or 2 μg of RFBL per ml. Cytokine levels (mean ± SD) in culture supernatant fluids from cells of individual mice (n = 4) were determined by ELISA. One of three separate experiments with similar results is depicted.