Figure 3.
Migration of macrophage cells. In serum-free DMEM media with TCDD (10−8 M), IP (10−7 M) and FICZ (10−7 M), RAW 264.7 cells were cultured for 24 h, and images were taken. Crystal violet-stained cells take on a purple hue. The quantification of cells was performed as described above. The number of cells that migrated were totaled and presented as folds of increase compared to the control cells. Five independent experimental means ± SEM are represented in the graph. Unstimulated comparisons to control cultures were depicted by asterisks, such as. ** p < 0.01 by one-way ANOVA analysis.