Figure 5.
Effect of conditioned medium. In serum-free DMEM media with TCDD (10−8 M), IP (10−7 M), FICZ (10−7 M) for 24 h, RAW 264.7 cells were maintained. MLE-12 cells were seeded for 10–12 h before treatment for 24 h with RAW 264.7 conditioned medium. Images were taken after 24 h. Crystal violet-stained cells took on a purple hue. The quantification of cells was performed as illustrated previously. The number of cells that migrated were totaled and presented as folds of increase compared to the control cells. Five independent experimental means ± SEM were represented in the graph. Unstimulated comparisons to control cultures were depicted by asterisks, such as *** p < 0.001 and **** p < 0.0001 by one-way ANOVA analysis.