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. 2022 Nov 9;23(22):13805. doi: 10.3390/ijms232213805

Figure 3.

Figure 3

LPS increased UCP1 expression in brown adipocytes while downregulating miR-143. (A) The miR-143 and Ucp1 mRNA expression levels in the isolated SVF cells and mature adipocytes from BAT of mice treated with or without 10 μg/kg LPS (n = 6); nd., not detected. (B,C) The miR-143 and Ucp1 mRNA expression in primary brown adipocytes in response to 24 h treatment with various concentrations of LPS (n = 6). (D,E) Time course of miR-143 and Ucp1 mRNA induction in primary brown adipocytes in response to LPS (0.2 μg/mL) treatment (n = 6). (F–H) The miR-143 expression (G) and UCP1 protein levels (H) in WT and miR-143-overexpressed primary brown adipocytes treated with or without 0.2 μg/mL LPS. Data are presented as the mean ± SEM. * p < 0.05 vs. controls; ** p < 0.01 vs. controls. Different letters above bars represent significant differences, with shared letters representing no significant differences, as determined by a two-tailed unpaired Student’s t-test (A–F) or two-way ANOVA followed by Tukey’s post hoc test (G).