Figure 3.

Effects of various metal chelators on naringin-induced apoptosis in four distinct cancer cell lines. MDA-MB-231, BxPC3, MDA-MB-468, and C42B cancer cells were treated with 100 µM naringin either alone or in the presence of the copper chelator neocuproine (Neo), the iron chelator desferrioxamine mesylate (DM), or the zinc chelator histidine (His), as shown in the figure. The metal chelator concentration utilized was 50 µM. As mentioned in the Materials and Methods section, ELISA was conducted 72 h following treatment. Values reported are ±S.E.M. of three independent experiments. * p-value < 0.05 when compared to respective control.