Figure 4.

The effect of naringin on the elevated mRNA transcript levels of copper transporters CTR1 and ATP7A in MCF-10A-Cu cells compared to MCF-10A cells. Total RNA was extracted utilizing TRIzol reagent (Invitrogen, Carlsbad, CA, USA) per manufacturer’s instructions. CTR1 and ATP7A mRNA expression was quantified using real-time PCR, as stated in the Materials and Methods section. To determine the effect of naringin on mRNA expression, only MCF-10A-Cu (normal MCF-10A cells grown in a medium containing 25 µM CuCl₂) was treated with 100 µM naringin. Values reported are ±S.E.M. of three independent experiments. * p-value < 0.05 when compared to untreated control.