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. 2022 Oct 29;14(11):2402. doi: 10.3390/v14112402

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) PCA plot (PC1 and PC2) of the naïve library showing four clusters in the pool of sequences. (B) Heatmap showing overrepresentation (red) or underrepresentation (blue) compared to the expected value of each amino acid for each position according to the expected value for cluster 1. (C) Heatmap showing overrepresentation (red) or underrepresentation (blue) compared to the expected value of each amino acid for each position according to the expected value for cluster 2, cluster 3, and cluster 4.