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Overexpression and purification of recombinant β-galactosidase of L. bulgaricus 43 in E. coli BL21(DE3), demonstrated by SDS-PAGE in 10% separating gel after silver staining. Legend: (1) PerfectTM Tricolor Protein Ladder; (2) crude extract of the cells of untransformed E. coli BL21(DE3) as control; (3) crude extract from E. coli BL21(DE3) cells, bearing pET-41-β-gal and induced with 1 mM IPTG for 24 h; (4) the purified enzyme β-galactosidase.
