Table 2.

Primers used in this study.

Primer	Sequence (5′-3′) 1	Positions in β-gal 2	Purpose
LacZ_F	ATGAGCAATAAGTTAGTAAAAGAAAAAAG	1–29	Sequencing
LacZ_R	TTATTTTAGTAAAAGGGGCTGAATCAC	3000–3027	Sequencing
LacZ_FF	GTGAAGGTGACTTGGTTGCTGAAAA	803–828	Sequencing
LacZ_RR	CCAGAAGGTAAATTCCGGCAGCCGCTTC	2285–2313	Sequencing
LacZ_Nde	CAGTCCATATGATGAGCAATAAGTTAGTAAAAGAAAAAAG	1–29	Cloning
LacZ_Xho	CTAGTCTCGAGTTTTAGTAAAAGGGGCTGAATCAC	3000–3024	Cloning

¹ The underlined sequences are sites recognized by endonucleases. ² NCBI GenBank acc. no OP.