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Effects of the storage temperature for in vitro luciferase assay. The mRNA-LNP storage was preserved at −80, −30, 4, and 25 °C for 7 days and thawed. Firefly luciferase expression in HepG2 cells was measured post 24 h adding of each mRNA-LNPs. Data are represented as mean + S.D. (n = 3). * p < 0.05, ** p < 0.01 indicate significant differences (Tukey’s test).
