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Effects of the cryoprotectants against freeze-thaw at −80 °C for in vitro luciferase assay. The mRNA-LNP storage was preserved at −80 °C (added sucrose or not) or 4 °C for 7 days and thawed. Firefly luciferase expression in HepG2 cells was measured post-24 h, adding each mRNA-LNPs. Data are represented as mean + S.D. (n = 3). ** p < 0.01 indicate significant differences (Tukey’s test).
