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The effects of C29, TAK242 on BRHE induced NO, IL-1b, IL-6, and TNF-α production. The RAW 264.7 cells (A) and peritoneal macrophages (B) were pre-treated with C29 (TLR2 inhibitor), TAK242 (TLR4 inhibitor) for 30 min and then treated with BRHE (200 μg/mL) for 20 h. Representative pictures of Griss assay, ELISA, and quantitative analyses. Data are expressed as mean ± SD of three independent experiments. ## p < 0.01 versus control; ** p < 0.01 versus BRHE treatment alone.
