Table 3.
Detection of CNV in 22 genes suitable as potential pharmacoresistance and pathogenicity biomarkers in L infantum. Variation in its local copy number for each sample, ranging between −1 and +7 gene copies compared to the diploid dotation (0). * Tetrasomy in chromosome 31 must be considered.
| MHOM/TN/80/IPT-1 | MHOM/ES/2016/CATB101 | MCRI/ES/2006/CATB033 | LCAN/ES/2020/CATB102 | ||
|---|---|---|---|---|---|
| Miltefosine transporter and associated genes | CNV | CNV | CNV | CNV | |
| LinJ.13.1590 | LdMT | 0, +1 | 0, +1 | −1 | 0 |
| LinJ.13.1600 | Hypot. Protein | +1 | −1 | 0 | 0 |
| LinJ.32.1040 | ldRos3 | −1 | 0 | −1 | 0 |
| Miltefosine sensitivity locus (MSL) | CNV | CNV | CNV | CNV | |
| LinJ.31.2370 * | LinJ.31.2370 | +4 | +1, +2 | +2 | +2 |
| LinJ.31.2380 * | LinJ.31.2380 | +4 | +1, +2 | +2 | +1, +2 |
| LinJ.31.2390 * | LinJ.31.2390 | +4, +5 | +1, +2 | +2 | +1, +2 |
| LinJ.31.2400 * | LinJ.31.2400 | +3 | +2 | +2 | +1, +2 |
| METK locus | CNV | CNV | CNV | CNV | |
| LinJ.30.3550 | Lorien protein | 0 | +1, +2 | +1 | 0 |
| LinJ.30.3560 | METK1 | −1 | +1 | +1 | 0 |
| LinJ.30.3570 | Lorien protein | −1 | +1 | 0, +1 | −1 |
| LinJ.30.3580 | METK2 | 0 | 0, +1 | 0, +1 | 0 |
| H locus | CNV | CNV | CNV | CNV | |
| LinJ.23.0280 | YIP1 | +6, +7 | +1 | 0, +1 | +1, +2 |
| LinJ.23.0290 | MRPA | +5 | +1, +2 | 0, +1 | +1, +2 |
| LinJ.23.0300 | LinJ.23.0300 | +5 | +2 | 0 | 0 |
| LinJ.23.0310 | PTR1 | +4 | 0, +1 | −1 | +1 |
| AQP1 | CNV | CNV | CNV | CNV | |
| LinJ.31.0030 * | AQP1 | +5 | +1 | +1, +2 | +2, +3 |
| MAPK1 | CNV | CNV | CNV | CNV | |
| LinJ.36.6760 | LMPK | −1 | 0 | −1 | −1 |
| Amphotericin | CNV | CNV | CNV | CNV | |
| LinJ.2510 | SMT | 0 | 0, +1 | 0, +1 | 0 |
| Paramomycin-resistant locus | CNV | CNV | CNV | CNV | |
| LinJ.27.1940 | D-LDH | 0 | 0 | 0 | 0, +1 |
| LinJ.27.1950 | B-CAT | −1 | 0, +1 | 0 | 0, +1 |
| LACK antigen | CNV | CNV | CNV | CNV | |
| LinJ.28.2940 | LACK1 | 0, +1 | 0, +1 | 0 | 0 |
| LinJ.28.2970 | LACK2 | 0, +1 | 0, +1 | 0 | 0, +1 |