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Tricine SDS-PAGE analysis of the different clones expressing the recombinant peptides. MM: molecular marker (kDa). The figure shows the 16% tricine SDS-PAGE analysis of the expression of recombinant Vm24 (a), AnTx (b), and Ts6 (c) by the clones growing at 2000 µg/mL zeocin (numbers above the lines refer to the clone numbers). The red circle indicates the selected clones that showed the highest protein expression.
