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. 2022 Nov 17;8(11):1215. doi: 10.3390/jof8111215

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RP-HPLC purification of Vm24, AnTx, and Ts6. (a) RP-HPLC chromatograms of the recombinant peptides. Chromatograms show the fractions that are biologically active on the hKv1.3 channel. Acetonitrile % of elution and retention time for recombinant peptides were: Vm24 23.4%/31.8 min, AnTx 21.2%/32.7 min, and Ts6 25.9%/36.8 min. The inset in each chromatogram represents an ESI-QTOF-MS spectrum showing the monoisotopic mass of the respective fraction. (b) Sixteen percent tricine SDS-PAGE of the purified peptides. MM: molecular marker (kDa), line 1: Vm24, line 2: Ts6, and line 3: AnTx.