Figure 5.

Blocking kinetics of recombinant Vm24, AnTx, and Ts6 on hKv1.3. Whole-cell currents through hKv1.3 were evoked from activated human peripheral T lymphocytes by depolarization to +50 mV from a holding potential −120 mV for 15 ms duration. Test pulses were applied every 15 s. Time courses of the development and recovery of the hKv1.3 current inhibition are shown for each peptide. Normalized peak K⁺ currents (I/I₀) were determined and plotted as a function of time. Down and up arrows indicate the start and the end of perfusion with the recombinant peptide containing solution or toxin-free solution, respectively. Data points in blue, green, or purple shades represent the addition application of recombinant Tag-Vm24 (panel (a,b)), AnTx (panel (c,d)), and Ts6 (panel (e,f)), respectively, in their tagged or untagged version, as indicated.