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. 2022 Nov 9;12(11):1121. doi: 10.3390/membranes12111121

Figure 5.

Figure 5

Representative microscopic images of multicomponent liposomes made with ergosterol as the sterol component, featuring three different phase-separated lipid domains (A,B), and percentages of phase-separated structures (D). (A) Homogenous (DPPG:DPPC:DOPC:Erg), 15:25:40:20 made in pure water (PW); (B) Lo/Ld (DPPC:DOPC:Erg), 40:40:20 made in pure water (Control), and (C) So/Ld (DPPG:DPPC:DOPC:Chol), 15:25:40:20 made in a sodium chloride (NaCl) solution at 20 °C. These images were taken by the author. (D) The percentages of phase-separated structures (homogenous, Lo/Ld, and So/Ld) in DPPC:DOPC:Erg; 40:40:20, (Control, bright grey); DPPG:DPPC:DOPC:Erg (15:25:40:20) made in pure water (PW, grey), and DPPG:DPPC:DOPC:Erg (15:25:40:20) made in a NaCl solution (NaCl, dark grey), respectively, at 20.0 °C. Condition PW means the number ratio of homogeneous, Lo/Ld, So/Ld liposomes observed in pure water and condition NaCl means those of observed in a 10 mM of NaCl solution. Each bar represents the average value of three samples; standard errors are shown as error bars. Abbreviations: DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; DPPG, 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol; Chol, cholesterol; Lo/Ld, liquid-ordered/liquid-disordered; So/Ld, solid-ordered/liquid-disordered.