Fatty acid composition of astrocytes of BBB and BBB-H model at control conditions (0) and 20 µM HNE treatment based on the degree of saturation: saturated fatty acids (SFA) composition (A); monounsaturated fatty acids (MUFA) composition with major representatives (B,C); polyunsaturated fatty acids (PUFA) composition with major ω3 and ω6 representatives (D–F). All results are represented as mean ± SEM, n = 4 samples for each group from 2 independent experiments. a—statistically significant in respect to control (0) on pure collagen, p < 0.05; b—statistically significant in respect to control (0) on modified collagen, p < 0.01; c—statistically significant between groups of same treatment, p < 0.01.