Table 2.
RTT tumor BRCA1/2 analysis strategies.
| Laboratory | Chemistry | NGS-Panel | NGS-Instrument |
|---|---|---|---|
| Lab1 | Multiplex-PCR | Oncomine Comprehensive Assay v3 (Thermo Fisher) | Ion S5™ System (Thermo Fisher Scientific) |
| Lab2 | Multiplex-PCR | BRCA MASTR Plus Dx (Multiplicom) | MiSeq (Illumina) |
| Lab3 | Hybrid Capture | Sure Select XT (Agilent) | Ion S5™ System (ThermoFisher Scientific) |
| Lab4 | Hybrid Capture | MiniHRS (Sophia Genetics) | MiSeq (Illumina) |
| Lab5 | Multiplex-PCR | Oncomine BRCA Research Assay (Thermo Fisher) | Ion S5™ System (Thermo Fisher Scientific) |