Table 2.

Amino acid substitutions of the ERG11 gene identified in cultures of C. albicans CAF2-1 strain under various conditions. Hot-spot amino acid mutations are marked in bold; previously identified mutations are marked in italics; mutations that did not result in an amino acid substitution are underlined.

			Concentration of Fluconazole [µg/mL]
			0	0.25	1	4	16
Carbon source	Glucose	8 h	N273K, L276V	I11V, Y13N, N273K, L276V	L35F, A249D, K251STOP, L276V, W520R	N12I, Y13N, E266G, L276V, W520R	I11V, Y13N, K128T, D275G, L276V, W520R
		14 h	N12I, Y13N, R264E, R265K	N12I, Y13N, L276V	N12I, L35F, A249D, L276V	I11M, N12I, K128T, Q250H, L276V, W520R	D9E, N12Y, K119N, K128T, L276V, W520R
		24 h	I11A/I11G, F31V, L276V	I11A/I11G, D275R/D275C, L276V	I11M, N12I, Y13N, D225H, L276V, S506G	I11A/I11G, R265K, E266R, L276V, S506G	I11T/I11S, N12I, L276V, N490K, S506G
	Lactate	8 h	I11A/I11G, L276V	I11S/I11R, N12Y, Y13N, R267S/R267G, L276V	I11A/I11G, L276V	G10D/G10V, N12I, Y13N, L276V, N490K	I11L/I11V, N12L, L276V
		14 h	I11M, N12Y, R265G, R265K, L276V	I11M, N12Y, L276V	N12Y, D225Y, L276V	N12Y, K128T, L276V	D9Q, N12Y, K128T, K259I, R265G, L276V
		24 h	I11A/I11G, R265G, L276V	K259I, R265G, L276V	I11V, R265G, L276V	I11A/I11G, K128T, E260D, K262I, L276V	G10S, N12I, K128T, E260D, E266K, R267S, L276V, E517D