Table 4.

Description of the ZL-ERG11A and ZL-ERG11B programs used for the amplification of “A” and “B” fragments of the ERG11 gene.

Step	Program				Cycles
	ZL-ERG11A		ZL-ERG11B
	Time	Temperature	Time	Temperature
Initial denaturation	5 min	98 °C	5 min	98 °C	-
Denaturation	30 s	98 °C	28 s	98 °C	35
Annealing	30 s	51 °C	29 s	55 °C
Extension	30 s	72 °C	30 s	72 °C
Final extension	5 min	72 °C	5 min	72 °C	-
	∞	4 °C	∞	4 °C	-