Figure 6.

PI(4,5)P₂ diffusion is uninhibited at clathrin-coated structures. (A) Schematic of diffusion in the PM proximal to clathrin-coated structures contact sites marked with endogenously tagged sfGFP-Clathrin light chain. (B) TIRF image of a small region of PM from an edited sfGFP-CLC cell; insets show a region of raw and thresholded images overlaid with single-molecule trajectories. Inset = 1.5 µm. (C) Diffusion coefficients, α (from msd v ∆t plots as well as fast and slow populations), the fraction of the population with slow diffusion, and mean turning angles are shown for trajectories classified as inside or outside ER–PM contact sites. Data are the grand means ± 95% C.I. of nine cells. *P ≤ 0.05 from paired t test with Holm–Šidák correction for multiple comparisons.