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. 2022 Nov 16;23(22):14174. doi: 10.3390/ijms232214174

Figure 7.

Figure 7

ERS is involved in regulating the E. coli-induced inflammatory response in bEECs. Primary bEECs were either uninfected or infected with E. coli for 6 h after pretreatment with EGTA (5 mM), NAC (8 mM), BAPTA-AM (10 μM), or 2-APB (50 μM). (A) Western blot analysis of ERS-related proteins (GRP78, p-IRE1, p-eif2α, and ATF6) during E. coli infection for 1–6 h. (B) Expression of ERS-related proteins (GRP78, p-IRE1, p-eif2α, and ATF6) measured by Western blot after 6 h of infection. The data are means ± SEM of three independent experiments. Bars with different letters indicate significant differences (p < 0.05).