. 2022 Nov 19;11(22):3171. doi: 10.3390/plants11223171

Table 2.

Information on the molecular markers employed.

Code	Locus	(Name) Primer Sequence 5′-3′	Ta (°C)	Ref.
cpDNA
Pr1	trnQ^(UUG)-psbK IGS	(F: trnQ-IGSR)^§ ACC CGT TGC CTT ACC GCT TGG (R: psbK-IGSR)^§ ATC GAA AAC TTG CAG CAG CTT G	58	1
Pr8	trnS^(UGA)-psbZ IGS	(F: psbZ-IGS) AAT AGC CAA TTG AAA AGC (R: trnS_UGA-IGSR)^§ ATC AAC CAC TCG GCC ATC	55	1
Pr11	ycf3 intron-1	(F: ycf3-E1F)^§ CAT TTA CCT ATT ACA GAG ATG G (R: ycf3-E2R) TTC CGC GTA ATT TCC TTC	50	1
Pr18	trnV^(UAC)-atpE IGS	(F: atpE-IGSF)^§ AGT GAC ATT GAT CCR CAA GAA GC (R: trnV_UAC-E1R) GTG TAA ACG AGG TGC TCT AC	57	1
Pr38	psbA-trnH^(GUG) IGS	(F: psbA3f) GTT ATG CAT GAA CGT AAT GCT C (R: trnHf)^§ CGC GCA TGG TGG ATT CAC AATCC	55	2, 3
nrDNA
N43	ITS1+5.8S+ITS2	(F: JK14)^§ GGA GAA GTC GTA ACA AGG TTT CCG (R: SN3)^§ TTC GCT CGC CGT TAC TAA GGG	55	4, 5

IGS = intergenic spacer; F = forward primer; R = reverse primer; Ta = annealing temperature; ^§ = primer used for sequencing; Ref. = reference, 1 = [63]; 2 = [64]; 3 = [65]; 4 = [66]; 5 = [67].