TABLE 4.
Strain | Serotype | Size of PCR product (bp) from regiona:
|
|||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
I | II | III | IV | V | VI | VII | VIII | IX | X | XI | XII | XIII | XIV | XV | XVI | XVII | XVIII | ||
STEC 3172/97 | O128:H2 | 1,200 | 1,400 | 1,200 | 160 | 800 | 230 | 280 | 240 | 360 | 780 | —b | 800 | 2,800 | 2,800 | 1,300 | 300 | 1,700 | 2,500 |
STEC 5720/96 | O26:H− | 900 | 1,100 | 900 | 160 | 800 | 230 | 280 | 240 | 360 | 780 | — | 800 | 2,800 | 2,800 | 1,300 | 300 | 1,700 | 2,500 |
E. coli DH5α | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | |
Y. pestis KIM6+ | 1,255 | 1,500 | 1,203 | 160 | 797 | 233 | 286 | 237 | 359 | 780 | 100 | 830 | 2,797 | 2,805 | 1,340 | 300 | 1,762 | 2,518 | |
Y. enterocolitica WA-314 | O8 | 1,255 | 1,500 | 1,203 | 160 | 797 | 233 | 286 | 237 | 359 | 780 | NPc | 830 | 2,797 | 2,930 | 1,340 | 300 | 1,762 | 2,518 |
I to XI are regions shown in Fig. 1A; XII to XVIII are regions shown in Fig. 1B. The sizes of Yersinia PCR products derived from regions I to XVIII are according to the published HPI sequences (13, 34, 39); the sizes of E. coli PCR products derived from the corresponding regions of E. coli strains were determined after agarose gel electrophoresis.
—, no PCR product obtained.
NP, not present.