Table 4.
Reactive flavivirus-ELISA results of raccoon/raccoon dog serum samples and differentiation between WNV, USUV and TBEV by neutralization tests. Samples were considered positive (POS) by blocking ELISA when the inhibition percentage (IP) was ≥40% and doubtful (DOUBT) by IP > 30% to <40% and IP ≤ 30% as negative (not shown). Positive ELISA and neutralization assay results are shown in bold; questionable ELISA results and cross-reacting antibody titers are also displayed.
| Blocking ELISA | Differentiation by Virus Neutralization Tests | ||||
|---|---|---|---|---|---|
| FLI ID-Number | Inhibition Percentage (IP%) | Result | WNV Antibody Titer (ND50) | USUV Antibody Titer (ND50) | TBEV Antibody Titer (ND50) |
| 1-W-B-142 | 93.02 | POS | 1920 | 160 | <10 |
| 1-W-B-155 | 94.64 | POS | 2560 | 60 | <10 |
| 1-W-B-171 | 91.56 | POS | 120 | <10 | 15 |
| 1-W-B-174 | 94.33 | POS | 640 | 30 | <10 |
| 1-W-B-180 | 94.22 | POS | 2560 | 480 | <10 |
| 1-W-B-190 | 93.15 | POS | 40 | 10 | <10 |
| 1-W-B-201 | 94.71 | POS | 960 | 20 | <10 |
| 1-M-B-006 | 86.44 | POS | <10 | 80 | <10 |
| 1-W-B-033 | 85.71 | POS | <10 | 40 | <10 |
| 1-W-B-153 | 34.87 | DOUBT | <10 | 80 | <10 |
| 1-W-B-178 | 44.12 | POS | 10 | 80 | <10 |
| 1-M-B-004 | 89.74 | POS | 30 | 15 | 120 |