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EspB is required for cell association by C. rodentium. The number of adherent bacteria on 50 HEp-2 cells was counted. Cell association of wild-type DBS100 was not significantly different from that of espB+ DBS587 (P = 0.05). The espB mutant DBS578, like eaeA mutant DBS255, had reduced cell adherence (P < 0.001). The trans-complemented DBS578/pJVN111 strain had increased cell association compared to wild-type DBS100 (P < 0.001).
