Figure 2.

Predicted and observed effects of the SCFAs mixtures. (I,II) Predicted and observed effects of the SCFAs mixtures I (A) and II (B) of the three tested SCFAs in RKO (Aa,Ba), HCT-15 (Ab,Bb) and NCM460 (Ac,Bc) cells by SRB assay. Individual data points are represented by black circles, and the best-fit regression model is shown by the black line, labeled “observed effect”. Black dashed lines represent the upper and lower 95% CI for the regression fit. The solid red line shows the prediction according to concentration addition (CA). The blue dotted lines show the IC₅₀ for each response curve (for values see Table 2). Experimental data were obtained from a minimum of three independent experiments run in triplicate.