Figure 6.

Energetic metabolism effects induced by SCFAs, individually or in mixture, in CRC cells. (a–d) Lactate-production quantification using the Lactate LO-POD enzymatic colorimetric kit from Spinreact in CRC cells treated with each SCFA, individually or in mixture, for 48 h. (a) RKO cells were incubated with the IC₅₀ of each SCFA (81.04 mM of acetate, 10.84 mM of butyrate, 32.25 mM of propionate). (b) RKO cells were incubated with the IC₅₀ of the mixture II, as well as the correspondent concentrations of each SCFA in that mixture (47.61 mM of Mix II containing 28.57 mM of acetate, 7.14 mM of butyrate, and 11.90 mM of propionate). (c) HCT-15 cells were incubated with the IC₅₀ of each SCFA (89.52 mM of acetate, 4.57 mM of butyrate, 22.70 mM of propionate). (d) HCT-15 cells were incubated with the IC₅₀ of the mixture II, as well as the correspondent concentrations of each SCFA in that mixture (13.67 mM of Mix II containing 8.20 mM of acetate, 2.05 mM of butyrate, and 3.42 mM of propionate). Cells were incubated with fresh complete medium as negative control. Data are from at least three independent experiments run in triplicate. Statistical analysis was performed using Two-Way ANOVA (p-value 0.0332 (*), 0.0021 (**), 0.0002 (***) and <0.0001 (****)).