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Confirmation of mucus-binding factor knockout Lacticaseibacillus rhamnosus CRL1505 (ΔmbfCRL1505) strain obtention by Western Blot analysis. Western blotting using a specific antibody to the mbf of L. rhamnosus (rabbit anti CRYVRLAADSAAASGTFPKD). M: Protein molecular weight marker (Promega). CRL1505: 45 KDa band belonging to the mbf protein of the wild-type L. rhamnosus CRL1505 strain. ΔmbfCRL1505: Absence of the mbf protein in the mutant CRL1505 strain.
