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. 2022 Nov 11;11(11):1330. doi: 10.3390/pathogens11111330

Figure 1.

Figure 1

Interferon-γ (panel (A)) and delayed type hypersensitivity (DTH, panel (B)) responses upon aerosol M. bovis Ravenel (n = 3) infection. Panel A. Whole blood cultures were stimulated with 20 mg/mL M. bovis PPD (Prionics AG) or medium alone (no stimulation) for 48 h. Stimulated plasma were harvested, and IFN-γ concentrations were determined by ELISA (Bovigam, Prionics, Ag). Values represent mean (±standard error) responses to antigen minus the response to media alone (D OD) for M. bovis Ravenel-infected cattle. Response kinetics to rESAT-6/CFP10 were similar to M. bovis PPD responses (data not shown). * Differs (p < 0.05) from pre-challenge (Day 0) responses. Panel B. Approximately 2 months after challenge, 0.1 mL (100 mg) of M. bovis PPD and 0.1 mL (40 mg) of M. avium PPD were injected intradermally at separate clipped sites in the mid-cervical region of each calf. Values represent mean (±standard error) change in skin thickness (i.e., 72 h post-injection minus pre-injection).