Table 3.
Predicted BoLA-I presented T. parva peptides identified in this study. For each peptide, the accession number and description of the protein from which it is derived are shown in columns A and B, and specific comments about particular proteins are given in column C. Columns D–H provide details about the individual peptides, including their sequence, length, the sample(s) they were identified in, the BoLA-MHCI allele predicted to present the peptide, and the predicted percent rank binding score.
| Accession | Protein Description | Comments | Peptide | Length | Sample | BoLA_MHCI Allele | %Rank |
|---|---|---|---|---|---|---|---|
| TpMuguga_01g00075 | hypothetical protein | NQPKNVVEF | 9 | 5350TP_rpt | 3:02701 | 0.04 | |
| TpMuguga_01g00100 | Protein arginine N-methyltransferase 5 | SEIDVKDVL | 9 | 2824TP_rpt | 6:01401 | 0.01 | |
| TpMuguga_01g00151 | Insulinase (Peptidase family M16) family protein | SEVAVSAMGPL | 11 | 2824TP_rpt | 6:01401 | 1.87 | |
| TpMuguga_01g00176 | putative integral membrane protein | VEDEAAYHVQL | 11 | 2824_TP | 2:01601 | 0.03 | |
| TpMuguga_01g00188 | Tp6—Prohibitin-2 | IGSAIKDNPAFITL | 14 | 2229TP | 6:04001 | 1.66 | |
| TpMuguga_01g00235 | Eukaryotic translation initiation factor 3 subunit A | AEKEIVELV | 9 | 2123TP_rpt | 1:01901 | 0.01 | |
| TpMuguga_01g00386 | DNA polymerase family A family protein | QAYQQKVDL | 9 | 2229TP | 2:02501 | 0.45 | |
| TpMuguga_01g00421 | RWD domain protein | Identifications in A10 and A18 samples | SSISSSLLSVK | 11 | 1011TP | 2:01201 | 0.05 |
| TpMuguga_01g00421 | RWD domain protein | Identifications in A10 and A18 samples | KLIWRFIRHL | 10 | 641TP_rpt | 6:01301 | 0.29 |
| TpMuguga_01g00461 | Cytokine-induced anti-apoptosis inhibitor 1, Fe-S biogenesis family protein | GQVTKASFFSSL | 12 | 641TP_rpt | 6:01301 | 0.23 | |
| TpMuguga_01g00471 | Bifunctional thioredoxin reductase/thioredoxin | Identification in three different A15 samples | FEYEFPINH | 9 | 2123TP_rpt/2408TP/2408_TP_rpt | 1:00901 | 0.03 |
| TpMuguga_01g00566 | Brix domain protein | NKKRPISIGF | 10 | 5350TP_rpt | 2:02601 | 0.13 | |
| TpMuguga_01g00736 | hypothetical protein | Identification in duplicate A18 samples | RMDDKSGGLL | 10 | 641TP | 6:01301 | 1.20 |
| TpMuguga_01g00757 | Ras family protein | Identification in duplicate A18 samples | GEFEKKYIPTL | 11 | 641TP | 6:01301 | 0.15 |
| TpMuguga_01g00808 | Ubiquitin elongating factor core family protein | SKKDLFIQF | 9 | 5350TP_rpt | 3:02701 | 0.01 | |
| TpMuguga_01g00926 | Cofilin/tropomyosin-type actin-binding family protein | VEDHDEVRGALA | 12 | 2824TP_rpt | 2:01601 | 1.95 | |
| TpMuguga_01g00934 | Heat shock cognate 90 kDa protein | SQFVKYPIQL | 10 | 641TP_rpt | 6:01301 | 0.00 | |
| TpMuguga_01g01188 | Translation initiation factor IF-2 | NNPIGRVGF | 9 | 5350TP_rpt | 3:02701 | 0.10 | |
| TpMuguga_01g01198 | hypothetical protein | RALFDDLHRY | 10 | 2229TP | 4:02401 | 0.28 | |
| TpMuguga_01g01207 | Pre-mRNA-splicing factor srp2 | FGPINRIDF | 9 | 5350TP_rpt | 3:02701 | 0.90 | |
| TpMuguga_01g01210 | Mitochondrial carrier family protein | TQYERIKERL | 10 | 641TP | 6:01301 | 0.01 | |
| TpMuguga_01g02005 | Rab-GTPase-TBC domain protein | KLNEQKILSL | 10 | 641TP_rpt | 6:01301 | 0.08 | |
| TpMuguga_01g02030 | hypothetical protein | Identifications in A12 and A15 samples | EEIAHVLHY | 9 | 2123TP_rpt | 1:01901/1:00901 | 0.39/1.00 |
| TpMuguga_01g02175 | Sas10 C-terminal domain protein | YLHEFHNFI | 9 | 2123TP_rpt | 2:02501 | 1.69 | |
| TpMuguga_02g00113 | DNA replication licensing factor MCM6 | SSLLKLTNK | 9 | A10TP_rpt | 2:01201 | 0.01 | |
| TpMuguga_02g00123 | DEAD/DEAH box helicase | GQIQDVFKRL | 10 | 641TP | 6:01301 | 0.25 | |
| TpMuguga_02g00142 | Polyubiquitin | SKDEHKKLY | 9 | 2229TP | 1:02301 | 0.07 | |
| TpMuguga_02g00248 | Nucleolar GTP-binding protein 1 | HMFSGKRTL | 9 | 641TP_rpt | 6:01301 | 0.05 | |
| TpMuguga_02g00488 | ATP synthase subunit D | AEDFKSLVI | 9 | 2824TP_rpt | 2:01601 | 0.01 | |
| TpMuguga_02g00543 | putative integral membrane protein | KLANSKNVSL | 10 | 641TP_rpt | 6:01301 | 0.11 | |
| TpMuguga_02g00551 | 23 kDa piroplasm membrane protein | SKATDRLVV | 9 | 5350TP_rpt | 3:02701 | 0.22 | |
| TpMuguga_02g00613 | DEAD/DEAH box helicase | AKKITELGF | 9 | 5350TP_rpt | 2:02601 | 0.06 | |
| TpMuguga_02g00703 | putative integral membrane protein | three different identifications in one A20 sample | VKKLKESLL | 9 | 5350TP_rpt | 2:02601 | 0.02 |
| TpMuguga_02g00703 | putative integral membrane protein | three different identifications in one A20 sample | NKLGDPLTL | 9 | 5350TP_rpt | 3:02701 | 0.12 |
| TpMuguga_02g00703 | putative integral membrane protein | three different identifications in one A20 sample | YKPEGMEYPF | 10 | 5350TP_rpt | 3:02701 | 1.59 |
| TpMuguga_02g00706 | hypothetical protein | YQKNSNNPFM | 10 | 5350TP_rpt | 2:02601 | 1.11 | |
| TpMuguga_02g00718 | hypothetical protein | GKNSVLLQV | 9 | 5350TP_rpt | 2:02601 | 0.15 | |
| TpMuguga_02g00723 | hypothetical protein | RTFNDVSKRKH | 11 | 2408_TP | 1:00901 | 0.71 | |
| TpMuguga_02g00753 | chaperone protein DnaK | TQVGIKVY | 8 | 2408_TP | 1:00901 | 0.15 | |
| TpMuguga_02g00895 | Tp9—Hypothetical protein | two overlapping identifications in one A10 sample | (RDDLG)SSFTSGYTK | 9/14 | 1011TP | 2:01201 | 0.01/0.58 |
| TpMuguga_02g00896 | hypothetical protein | AQGDPVFL | 8 | 2408_TP | 2:02501 | 0.16 | |
| TpMuguga_03g00253 | hypothetical protein | Identifications in A10, A15 and A20 samples | LQSEVFPNY | 9 | 2408_TP | 1:00901 | 0.11 |
| TpMuguga_03g00253 | hypothetical protein | Identifications in A10, A15, and A20 samples | FNFSESKLTF | 10 | 5350_TP | 3:02701 | 1.38 |
| TpMuguga_03g00253 | hypothetical protein | Identifications in A10, A15, and A20 samples | LNTSIGGSL | 9 | A10_TP | 3:00201 | 1.00 |
| TpMuguga_03g00257 | hypothetical protein | SQNNRSEMSNL | 11 | 641TP_rpt | 6:01301 | 0.07 | |
| TpMuguga_03g00330 | hypothetical protein | SSMRDALNPPPTH | 13 | 2408_TP | 1:00901 | 1.16 | |
| TpMuguga_03g00388 | hypothetical protein | SQKRKNKPL | 9 | 641TP_rpt | 6:01301 | 0.01 | |
| TpMuguga_03g00469 | High mobility group protein homolog NHP1 | AKKDPNAPKRAL | 12 | 5350TP_rpt | 2:02601 | 0.13 | |
| TpMuguga_03g00478 | DEAD/DEAH box helicase family protein | YVGKAPTLW | 9 | 5350TP_rpt | 3:02701 | 1.32 | |
| TpMuguga_03g00507 | CCA tRNA nucleotidyltransferase mitochondrial | SKVDRVSL | 8 | 2229TP | 1:02301 | 0.09 | |
| TpMuguga_03g00544 | putative integral membrane protein | NVFPLILGK | 9 | A10_TP | 2:01201 | 0.08 | |
| TpMuguga_03g00577 | Cwf15/Cwc15 cell cycle control family protein | SQQPPSFLNDAVRTDFH | 17 | 2408_TP | 1:00901 | 0.52 | |
| TpMuguga_03g00655 | N4—Hypothetical protein | GVDVDQLLH | 9 | 2123TP_rpt | 1:00901 | 0.36 | |
| TpMuguga_03g00747 | DEAD/DEAH box helicase | SMKGKHELTL | 10 | 641TP | 6:01301 | 0.01 | |
| TpMuguga_03g00852 | Protein transport protein Sec61 subunit alpha | two different identifications in one A10 sample | SSMVMQLLAGSK | 12 | A10TP_rpt | 2:01201 | 1.27 |
| TpMuguga_03g00852 | Protein transport protein Sec61 subunit alpha | two different identifications in one A10 sample | KGTEFEGALISL | 12 | A10TP_rpt | 3:00201 | 1.43 |
| TpMuguga_03g00858 | Multiprotein-bridging factor 1c | Identifications in duplicate A10 samples | AGVELDTQKKFL | 12 | A10_TP | 3:00201 | 1.36 |
| TpMuguga_03g00861 | p150—Hypothetical protein | LGPILIYEDL | 10 | A10TP_rpt | 3:00201 | 0.10 | |
| TpMuguga_03g02030 | N-terminal region of Chorein, a TM vesicle-mediated sorter family protein | SSIDVNVKL | 9 | 1011TP | 3:00201 | 0.09 | |
| TpMuguga_03g02350 | RF-1 domain protein | KQVVRDAMVEQDML | 14 | 641TP | 6:01301 | 1.58 | |
| TpMuguga_03g02680 | putative integral membrane protein | FGIPLVTK | 8 | A10TP_rpt | 2:01201 | 0.38 | |
| TpMuguga_04g00144 | hypothetical protein | NSNELKDIK | 9 | A10TP_rpt | 2:01201 | 1.60 | |
| TpMuguga_04g00206 | hypothetical protein | IEDEVCKVI | 9 | 2824TP_rpt | 2:01601 | 0.09 | |
| TpMuguga_04g00229 | Pescadillo N-terminus family protein | SLMPKKHKRLL | 11 | 641TP_rpt | 6:01301 | 0.08 | |
| TpMuguga_04g00233 | Protein IWS1 homolog | SQMSRNIESKH | 11 | 2123TP_rpt | 1:00901 | 0.03 | |
| TpMuguga_04g00368 | 26S proteasome non-ATPase regulatory subunit 4 homolog | IGLIASAKL | 9 | A10TP_rpt | 3:00201 | 0.27 | |
| TpMuguga_04g00439 | hypothetical protein | NSQLRQKIRSM | 11 | 641TP_rpt | 6:01301 | 1.09 | |
| TpMuguga_04g00484 | OST-HTH Associated domain protein | SGINLGNVNSL | 11 | A10_TP | 3:00201 | 0.09 | |
| TpMuguga_04g00662 | putative integral membrane protein | DKKALTVAL | 9 | 5350TP_rpt | 3:02701 | 0.01 | |
| TpMuguga_04g00754 | hypothetical protein | SQFPRNPVDSLL | 12 | 641TP_rpt | 6:01301 | 0.01 | |
| TpMuguga_04g00785 | Helicase C-terminal domain | NNFNHSLL | 8 | 5350TP_rpt | 3:02701 | 0.28 | |
| TpMuguga_04g00790 | AP2-coincident C-terminal family protein | SQSEEIEKYLH | 11 | 2408_TP | 1:00901 | 0.07 | |
| TpMuguga_04g02060 | hypothetical protein | HQQDSQYYLTQH | 12 | 2408_TP | 1:00901 | 0.10 | |
| TpMuguga_04g02625 | Importin subunit alpha-6 | KQLRNENEI | 9 | 641TP_rpt | 6:01301 | 0.41 |