Figure 9.

Efficacy of treatment with GY971a (pANDMA) in a murine model of acute lung infection. Mice were infected with 1 × 10⁶ colony-forming units (CFUs) of planktonic PAO1. 5 min after infection, 4.5 mg/kg, 9 mg/kg and 18 mg/kg GY971a (pANDMA) or the vehicle was administered as an aerosol by Penn Century. After 6 h, mice bronchoalveolar lavage fluid (BALF) was collected and the lungs were excised, homogenized and plated on tryptic soy agar to determine the bacterial burden. Total cell (A), neutrophil (B) and macrophage (C) counts were performed in bronchoalveolar lavage fluid (BALF). Bacterial burden was evaluated in lung (D), BALF (E) and in total lung (F). Data are presented as the mean ± sem pooled from two independent experiments n = 10–12. Statistics were performed by one-way ANOVA with Dunnet’s multiple comparisons test, using Graph Pad Prism 9.0. * p < 0.05, *** p < 0.001, **** p < 0.0001 compared to vehicle.