Table 4.
Cytokine and chemokine concentrations in supernatant of lung homogenates following acute P. aeruginosa airway infection and GY971a (pANDMA) treatments. Data are expressed as mean values ± standard errors of the means (SEM) pooled from two independent experiments (N = 8–10). Statistical significance is determined by one-way ANOVA with Dunnett’s multiple comparisons test and significant differences between treatments and the vehicle are highlighted in gray, using Graph Pad Prism 9.0. * p < 0.05, ** p < 0.01 *** p < 0.001 compared to vehicle.
| Cytokine/Chemokine | Concentration pg × 700 µg−1 | |||
|---|---|---|---|---|
| Vehicle (H2O/DMSO 4%) |
4.5 mg/Kg GY971a | 9 mg/kg GY971a | 18 mg/kg GY971a | |
| IL-1α | 88.45 ± 10.30 | 65.95 ± 7.91 | 54.31 ± 7.29 * | 43.51 ± 3.03 ** |
| IL-1β | 9.21 ± 1.49 | 6.95 ± 1.05 | 4.91 ± 0.59 * | 4.89 ± 0.75 * |
| IL-2 | 6.27 ± 0.28 | 5.58 ± 0.30 | 5.76 ± 0.24 | 5.28 ± 0.20 * |
| IL-3 | 5.11 ± 0.40 | 4.58 ± 0.24 | 4.36 ± 0.41 | 3.67 ± 0.25 * |
| IL-4 | 1.29 ± 0.09 | 1.23 ± 0.12 | 0.93 ± 0.14 | 0.87 ± 0.11 * |
| IL-5 | 8.46 ± 0.68 | 8.13 ± 0.37 | 7.89 ± 0.63 | 7.26 ± 0.44 |
| IL-6 | 110.0 ± 27.68 | 64.30 ± 10.86 | 49.64 ± 9.34 * | 69.79 ± 8.27 |
| IL-9 | 38.58 ± 1.01 | 35.43 ± 1.74 | 32.17 ± 2.04 * | 29.64 ± 0.69 *** |
| IL-10 | 20.76 ± 0.95 | 17.55 ± 1.23 | 16.41 ± 1.56 * | 14.79 ± 1.05 ** |
| IL-12p40 | 71.29 ± 4.32 | 61.72 ± 5.31 | 52.88 ± 4.67 * | 45.67 ± 2.62 *** |
| IL-12p70 | 167.6 ± 16.08 | 123.7 ± 12.91 | 110.1 ± 13.58 ** | 94.45 ± 6.61 ** |
| IL-13 | 88.02 ± 3.81 | 82.53 ± 2.63 | 80.58 ± 4.30 | 78.14 ± 3.36 |
| IL-17A | 5.49 ± 0.63 | 4.27 ± 0.26 | 3.98 ± 0.30 * | 3.84 ± 0.17 * |
| Eotaxin | 1627 ± 160.8 | 1638 ± 124.4 | 1618 ± 87.33 | 1414 ± 65.28 |
| G-CSF | 444.1 ± 86.41 | 259.1 ± 36.89 | 297.8 ± 58.72 | 181.7 ± 19.88 * |
| GM-CSF | 48.66 ± 5.94 | 45.70 ± 1.52 | 33.60 ± 4.60 * | 42.55 ± 3.18 |
| IFN-γ | 30.10 ± 1.43 | 27.02 ± 1.51 | 23.76 ± 1.65 ** | 22.51 ± 0.96 ** |
| KC | 2413 ± 456.1 | 2085 ± 263.6 | 1486 ± 150.8 | 2048 ± 248.3 |
| MCP-1 | 1813 ± 322.6 | 1171 ± 136.4 | 979.0 ± 144.3 * | 692.2 ± 78.41 ** |
| MIP-1α | 186.9 ± 36.52 | 131.7 ± 23.94 | 107.0 ± 12.10 | 92.11 ± 10.33 * |
| MIP-1β | 291.2 ± 30.26 | 254.0 ± 28.16 | 219.8 ± 20.41 | 212.2 ± 15.66 |
| RANTES | 110.3 ± 10.35 | 123.0 ± 9.93 | 109.9 ± 9.72 | 101.9 ± 9.40 |
| TNF-α | 104.6 ± 9.05 | 93.45 ± 6.93 | 95.61 ± 11.04 | 84.06 ± 5.22 |