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. 2022 Nov 9;23(22):13774. doi: 10.3390/ijms232213774

Figure 2.

Figure 2

Fascaplysin inhibited A549 cells migration. (A) Wnt/β-Catenin signaling pathway-related proteins were detected by Western blotting. (B–E) Gray levels of Wnt/β-Catenin signaling pathway-related proteins were quantified using ImageJ and normalized to gray levels of GAPDH protein. (F) After A549 cells were treated with fascaplysin (2 μM) for 3 h, β-catenin protein expression levels were detected using immunofluorescence. (G–J) Detection of EMT regulatory proteins by Western blotting. (K,L) Immunofluorescence analysis of the levels of N-cadherin and E-cadherin in A549 cells. (M) A549 cell migration assays were performed using transwell inserts and representative images were randomly photographed. Analysis results represent mean ± SD, * p < 0.05, and ** p < 0.01, *** p < 0.001.