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. 2022 Nov 13;8(11):1195. doi: 10.3390/jof8111195

Table 2.

The successful applications of metagenomic techniques to diagnose fungal pathogens.

Plant Aim of Study Metagenomics Techniques References
Grape Determination of fungi and oomycetes in different phyllosphere samples Metabarcoding [52]
Grape Determination of soil and leaf-associated fungal microbiota mNGS-Ilumina [53]
Wheat Detection of fungal microorganisms in the wheat phyllosphere Microbiome Metabarcoding using ITS barcodes [54]
Grape Identification of fungal diseases on the vine trunk mNGS-Ilumina [55]
Maize Determination of fungal microbiota after harvest Metabarcoding [56]
Wheat Determination of fungal communities in wheat residues Metabarcoding [57]
Grapevine Determination of fungal disease agents associated with grapevine Metabarcoding [58]
Banana Investigation of the effect of variable soil microbiota on fusarium disease Metabarcoding [59]
Wheat, maize To determine Fusarium species in various plants PaCBio SMRT Sequencing [60]
Strawberry Determination of microbial communities in strawberry growing soils with different yields Amplicon Based Metagenomic [61]