Skip to main content
. 1999 Nov;67(11):6056–6066. doi: 10.1128/iai.67.11.6056-6066.1999

TABLE 1.

Primers used in RT-PCR

Molecule Oligonucleotide Temp (°C) Maximal no. of cycles PCR product size (bp) Reference
IL-1α Sense: CCACTACAAAATCTGGGCGATGCA 66 33 213 21
Antisense: CAGAAGAAGAGGATGTCACTCCTCG
Probe: TATACTTCCTGTAACTTTAAGAATCTC
IL-1β Sense: AAAGCAGTTCCCTGCCCACAGGCC 66 30 388 10
Antisense: TGGGTAACGGTTGGGGTCTACACT
Probe: TGCAACACCTGGGATGACTACAGTCTTGAG
icIL-1ra Sense: GGACTCCACACAACCCACAAGACC 64 35 511 40
Antisense: CTACTGGTCCTCCTGGAAGTAGAA
Probe: GACCCTCCCTATGGCTTCAGA
sIL-1ra Sense: GAAGGTCTTCTGGTTAACATCCCA 64 35 150 40
Antisense: ATGAGACCCTCCAGGAGCACCCGC
Probe: AGGCACCTAATCTCCCTCCTCCTC
γ-Actin Sense: CCATCGTGGGGCGTCCGCGGCA 62 26 268 28
Antisense: ATCTGGGTCATCTTCTCGCGGT
Probe: GCGACGAGGCCCAGAGCAAGCGTG