Table 1.

Primers and probes employed in the Real-Time PCR assays.

Virus Investigated	Primers and Probes	Sequences	Reference
FCV	FCV-for	5′-GTAAAAGAAATTTGAGACAAT-3′	Abd-Eldaim et al., 2009 [24]
	FCV-rev	5′-TACTGAAGWTCGCGYCT-3′
	FCV-probe	FAM-CAAACTCTGAGCTTCGTGCTTAAA TAMRA
FCOV	FCoV-for	5′-AGCAACTACTGCCACRGGAT-3′	Dye et al., 2008 [25]
	FCoV-rev	5′-GGAAGGTTCATCTCCCCAGT-3′
	FCoV-probe	FAM-AATGGCCACACAGGGACAACGC-MGB-3′
FHV-1	FHV-1-for	5′-GGACAGCATAAAAGCGATTG-3′	Helps et al., 2003 [26]
	FHV-1-rev	5′-AACGTGAACAACGACGCAG-3′
	FHV-1-probe	FAM-5′-AATTCCAGCCCGGAGCCTCAAT-MGB-3′
FeLV	FeLV-for	5′-TCCCCAGTTGACCAGAGTTC-3′	Pinches et al., 2007 [27]
	FeLV-rev	5′-GATGGCTCGTTTTATAGCAGAAAG-3′
	FeLV-probe	FAM-AATCCCCATGCCTCTCGCTTCTGTA-MGB-3′
FPV	FPLV/CPV-for	5′-ACAAGATAAAAGACGTGGTGTAACTCAAATGGGAAATACAGACTATAT-3′	Decaro et al., 2008 [28]
	FPLV/CPV-rev	5′-CAACCTCAGCTGGTCTCATAATAGT-3′
	CPV-probe	FAM-5′-ATGGGAAATACAAACTATAT-MGB-3′
	FPV-probe	VIC-5’-ATGGGAAATACAGACTATAT-MGB-3′
RVA	RV-JVK-for	5′-CAGTGGTTGATGCTCAAGATGGA-3′	Jothikumar et al., 2009 [29]
	RV-JVK-rev	5′-TCATTGTAATCATATTGAATACCCA-3′
	RV-JVK -probe	5′-FAM-ACAACTGCAGCTTCAAAAGAAGWGT-3′(BHQ)