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. 2022 Nov 15;14(11):2526. doi: 10.3390/v14112526

Figure 3.

Figure 3

Specificity of the RF-RT-RAA assay for PRRSV-2. (A) Amplification results of RF-RT-RAA through real-time fluorescence read-out. (B) Amplicons of RF-RT-RAA observed with the naked eye under blue light. The image was captured by a mobile phone camera. Curves or tubes 1–4: RF-RT-RAA detection results of PRRSV-2 strains VR-2332 (RNA, 388 ng/μL), JS07 (RNA, 305 ng/μL), JXA1 (RNA, 206 ng/μL), and SH1705 (RNA, 178 ng/μL), respectively; curves or tubes 5–10: RF-RT-RAA detection results of PRV (DNA, 215 ng/μL), PEDV (RNA, 226 ng/μL), PCV2 (DNA, 264 ng/μL), TGEV (RNA, 314 ng/μL), PPV (DNA, 185 ng/μL), and negative control, respectively; PRV, porcine pseudorabies virus; PEDV, porcine epidemic diarrhea virus; PCV2, porcine circovirus type 2; TGEV, transmissible gastroenteritis virus; PPV, porcine parvovirus.