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. 2022 Nov 15;14(11):2526. doi: 10.3390/v14112526

Table 1.

Primers and probe used in this work.

Primer/
Probe
Sequence (5′-3′) Position Product Size (bp)
ORF6 F1 CGCAAGTACATTCTGGCCCCTGCCCACCAC 316–345 161
ORF6 R1 CTGCCACCCAACACGAGGCCATTCAACCCG 447–476
ORF6 F2 GCCGCAAGTACATTCTGGCCCCTGCCCACCA 314–344 165
ORF6 R2 TTCTGCCACCCAACACGAGGCCATTCAACC 449–478
ORF6 F3 TTGTGCTTGCTAGGCCGCAAGTACATTCTG 301–330 174
ORF6 R3 GCCACCCAACACGAGGCCATTCAACCCGGG 445–474
Probe CGATTGCGGCAAATGATAACCACGCATTTG
/i6FAMdT//THF/G/iBHQ1dT/CCGGCGTCCCGGCT (C3-Spacer)
371–418