Table 1.
Primer/ Probe |
Sequence (5′-3′) | Position | Product Size (bp) |
---|---|---|---|
ORF6 F1 | CGCAAGTACATTCTGGCCCCTGCCCACCAC | 316–345 | 161 |
ORF6 R1 | CTGCCACCCAACACGAGGCCATTCAACCCG | 447–476 | |
ORF6 F2 | GCCGCAAGTACATTCTGGCCCCTGCCCACCA | 314–344 | 165 |
ORF6 R2 | TTCTGCCACCCAACACGAGGCCATTCAACC | 449–478 | |
ORF6 F3 | TTGTGCTTGCTAGGCCGCAAGTACATTCTG | 301–330 | 174 |
ORF6 R3 | GCCACCCAACACGAGGCCATTCAACCCGGG | 445–474 | |
Probe | CGATTGCGGCAAATGATAACCACGCATTTG /i6FAMdT//THF/G/iBHQ1dT/CCGGCGTCCCGGCT (C3-Spacer) |
371–418 |