Figure 8.
Pretreatment effects of pro-inflammatory cytokines in the ALS disease model cell line. (A) MT cell line was pretreated with 1 mM of choline, and 20 ng/mL of LPS and TNF-α either in the presence or absence of choline for 24 h. [3H]Choline uptake was carried out at a temperature of 37 °C and pH of 7.4 for 5 min. (B) Slc44a1 mRNA expression levels in MT cell line, after pretreatment with aforementioned pro-inflammatory cytokines. The data represents mean ± S.E.M (n = 3–4). * p < 0.05 and ** p < 0.01, significantly different from the control; # p < 0.05 and ## p < 0.01, significantly different from the respective controls, i.e., LPS and TNF-α.