Figure 3.

Inhibition of mTOR improved the survival markers in HG-treated H9c2 cells. The H9c2 cells were seeded in low glucose medium overnight. Next, the cells were transfected with siRNAs against Raptor/Rictor for 24 h, followed by ET-1 (20 nM) treatment for 3 h and by HG treatment for another 24 h. Protein expressions of survival/apoptosis markers p-Akt, Bcl-2, and Bax (A); antioxidant markers SOD2, catalase, and HO-1 (B); and mitochondrial/autophagy marker PGC1α and TFEB (C) were determined using western blot. Protein densitometry analysis was done using Image J software (NIH). N = 3; Mean ± S.E.M.; * p < 0.05, ** p < 0.01, *** p < 0.001.