Table 2.

Inter-species comparative study of the binding affinity of L2 and BNP to natriuretic receptors.

	Human NPRs		Rat NPRs		Mouse NPRs		Chicken NPRs
NPR-A	L2	BNP	L2	BNP	BNP	L2	L2	BNP
ΔG° (kcal.mol)	−25.4 *	−18.0	−24.0 *	−16.9	−22.5	−19.9	−19.0 *	−15.4
SEM	1.0	0.4	0.8	0.2	0.2	0.8	0.2	0.2
NPR-B	L2	BNP	L2	BNP	L2	BNP	L2	BNP
ΔG° (kcal.mol)	−20.6 *,†	−19.0 †	−24.0	−17.0	−23.9	−22.4	−23.1 *	−18.4
SEM	0.3	0.8	0.4	0.3	0.4	0.2	0.1	0.1
NPR-C	L2	BNP	L2	BNP	L2	BNP	L2	BNP
ΔG° (kcal.mol)	−23.4 *	−18.3 £	−15.5 *	−14.4	−23.0 *	−19.5	−20.1 *	−17.7
SEM	0.7	0.4	0.2	0.1	0.2	0.1	0.2	0.2

Data were generated by a molecular docking study using Autodock Vina 1.1.2. software. Natriuretic peptides (NPs) in each row were ranked in decreasing order of interaction with the corresponding natriuretic receptor (NPR) (i.e., the lower the interaction energy, the better the stability of the receptor–ligand complex). BNP, B-type natriuretic peptide; L2, Lebetin 2; NPR, natriuretic peptide receptor (NPR); NPR-A, natriuretic peptide receptor A; NPR-B, natriuretic peptide receptor B; NPR-C, natriuretic peptide receptor C. ΔG°, Gibbs free energy. N = 3 values/condition.*, p < 0.05 vs. corresponding BNP; †, p < 0.05 vs. corresponding NPR-A; £, p < 0.05 vs. corresponding NPR-B.