Table 3.
Intra-species comparative study of the binding affinity of L2 and BNP to natriuretic receptors.
| L2 | BNP | |||||
|---|---|---|---|---|---|---|
| Human | hNPR-A | hNPR-C | hNPR-B | hNPR-B | hNPR-A | hNPR-C |
| ΔG° (kcal.mol) | −24.6 | −20.9 † | −20.1 † | −16.2 | −15.8 | −14.1 |
| SEM | 1.0 | 0.7 | 0.6 | 0.4 | 0.5 | 0.1 |
| Rat | rNPR-A | rNPR-B | rNPR-C | rNPR-B | rNPR-A | rNPR-C |
| ΔG° (kcal.mol) | −23.3 | −17.04 † | −17.0 † | −16.0 | −14.9 £ | −13.6 £ |
| SEM | 1.0 | 0.4 | 0.3 | 0.2 | 0.5 | 0.0 |
| Mouse | mNPR-B | mNPR-C | mNPR-A | mNPR-A | mNPR-B | mNPR-C |
| ΔG° (kcal.mol) | −18.9 | −18.5 | −17.9 | −18.3 | −15.8 | −13.5 † |
| SEM | 0.4 | 0.5 | 0.4 | 1.0 | 0.2 | 0.1 |
| Chicken | chNPR-B | chNPR-A | chNPR-C | chNPR-B | chNPR-A | chNPR-C |
| ΔG° (kcal.mol) | −21.5 | −20.1 | −19.0 £ | −15.0 | −14.7 | −14.4 |
| SEM | 0.4 | 0.4 | 0.4 | 0.2 | 0.5 | 0.4 |
Data were generated by a molecular docking study using Autodock Vina 1.1.2. software. The natriuretic peptide receptors (NPR) of each line were ranked in decreasing order of affinity with the corresponding natriuretic peptide (L2 and BNP), i.e., the lower the interaction energy between the receptor and the ligand, the better the stability of the complex. BNP, B-type natriuretic peptide; L2, Lebetin 2; chNPR, chicken NPR; hNPR, human NPR; mNPR, mouse NPR; rNPR, rat NPR. ΔG°, Gibbs free energy. N = 3 values/condition. †, p < 0.05 vs. corresponding NPR-A; £, p < 0.05 vs. corresponding NPR-B.