Table 6.
Representative table of electrostatic and hydrophobic interactions between natriuretic receptors and their ligands (L2 and BNP).
| hNPR-A | hNPR-B | hNPR-C | hNPR-A | hNPR-B | hNPR-C | ||||
|---|---|---|---|---|---|---|---|---|---|
| L2 | Monomer 1 electrostatic interactions |
ASP2-ARG95(A) LYS18-ASP177(A) ASP20-ARG95(A) ARG21-TYR120(A) ARG21-GLU169(A) |
ASP2-LYS110(A) LYS18-TYR159 (A) LYS18-GLU163(A) LYS18-GLU167(A) ASP20-LYS110(A) ARG21-TYR81(A) ARG21-SER85(A) SER26-TYR56(A) |
LYS18-GLU176(A) LYS18-GLU180(A) ARG21-TYR125(A) ARG21-TYR168(A) ARG21-GLU172(A) SER26-LYS162(A) SER26-ARG165(A) |
BNP | Monomer 1 electrostatic interactions |
SER8-ARG178(A) ARG13-ASP177(A) LYS14-ASP177(A) ARG17-TYR120(A) ARG17-GLU169(A) LYS27-TYR154(A) LYS27-GLU187(A) ARG30-TYR154(A) ARG30-GLU169(A) |
ARG13-SER172(A) LYS14-GLU129(A) LYS14-GLU167(A) LYS14-ASP361(A) LYS14-ASP365(A) SER21-SER85(A) SER21-ARG156(A) SER22-ARG156(A) LYS27-TYR148(A) LYS27-ASP150(A) LYS27-TYR159(A) LYS27-TYR181(A) ARG30-TYR148(A) ARG30-GLU163(A) ARG30-GLU167(A) ARG30-TYR148(A) ARG31-TYR148(A) ARG31-TYR159(A) |
ARG13-ASP122(A) ARG13-GLU180(A) ASP16-ARG99(A) SER21-ARG165(A) SER22-ARG165(A) LYS27-TYR168(A) ARG30-TYR168(A) ARG30-GLU172(A) ARG30-GLU176(A) ARG31-TYR168 (A) |
| Monomer 1 hydrophobic interactions |
PHE15-PHE165(A) PRO11-MET173(A) CYS14-MET173(A) ILE19-VAL116(A) LEU28VAL59(A) |
PHE15-PHE166(A) PRO5-LEU174(A) PRO6-LEU174(A) PRO11-VAL176(A) LEU28-PRO82(A) VAL33-CYS53(A) |
PHE15-PHE169(A) LEU28-CYS62(A) LEU28-CYS90(A) LEU28-ALA93(A) LEU28-LEU163(A) |
Monomer 1 hydrophobic interactions |
PRO2-ALA138(A) PRO2-ALA139(A) PRO2-LEU179(A) CYS10-MET173(A) ILE18-VAL87(A) ILE18-ALA90(A) ILE18-ALA91(A) ILE18-ALA111(A) ILE24-ALA155(A) ILE24-PRO158(A) ILE24-ALA189(A) CYS26-ALA189(A) |
PRO2-LEU95(A) MET15-ALA168(A) LEU29-VAL180(A) |
PHE11-TRP347(A) CYS10-ALA362(A) |
||
| Monomer 2 electrostatic interactions |
LYS4-TYR88(B) LYS4-TYR156(B) LYS4-ASP160(B) LYS4-GLU161(B) LYS4-GLU162(B) |
LYS4-TYR181(C) LYS7-GLU184(C) LYS8-TYR181(C) LYS8-GLU184(C) ASP20-ARG152(C) ARG21-GLU155(C) ARG21-TYR81(C) ARG21-ASP155(C) |
LYS7-ASP195(B) LYS7-TYR188(B) LYS8-ASP191(B) LYS8-ASP197(B) LYS8-ASP200(B) ARG21-168TYR(B) |
Monomer 2 electrostatic interactions |
ARG17-ASP62(B) ARG17-TUR88(B) LYS27-TYR154(B) LYS27-GLU169(B) LYS27-GLU187(B) ARG31-TYR88(B) ARG31-TYR156(B) ARG31-GLU162(B) ARG31-GLU169(B) |
ASP16-ARG152(C) ARG17-TYR81(C) ARG17-ASP150(C) ARG17-THR153(C) ARG17-ASP155(C) ARG31-TYR81(C) ARG31-ASP155(C) ARG31-TYR159(C) |
ARG17-TYR92(B) ARG17-GLU164(B) SER21-ARG99(B) |
||
| Monomer 2 hydrophobic interactions |
PHE15-TYR154(B) PHE15-PHE165(B) PRO5-PRO158(B) PRO6-PRO158(B) PRO6-ALA189(B) CYS30-VAL116(B) CYS30-MET173(B) |
PHE15-TYR181(C) ILE22-LEU149(C) |
PHE15-TYR181(B) PHE15-PHE190(B) PRO10-187ILE(B) PRO10-207ALA(B) ILE22-163LEU(B) |
Monomer 2 hydrophobic interactions |
MET15-VAL59(B) MET15-PRO158(B) LEU24–MET173(B) VAL28-LEU186(B) LEU29-ALA155(B) |
ILE18-CYS53(C) ILE18-CYS79(C) ILE18-VAL80(C) ILE18-PRO82(C) ILE18-ALA151(C) LEU29-LEU149(C) |
VAL28-ILE187(B) LEU29-LEU156(B) LEU29-LEU171(B) LEU29-ILE187(B) |
The residues involved in electrostatic and hydrophobic interaction between the two natriuretic receptor monomers (1 and 2) and L2/BNP were determined using Protein Structure and Interaction Analyzer (PSAIA) at 4.5 Å. BNP, B-type natriuretic peptide; L2, Lebetin 2; NPR-A, natriuretic peptide receptor A; NPR-B, natriuretic peptide receptor B; NPR-C, natriuretic peptide receptor C.