Figure 5.

Effect of coffee on gut propulsive motility and smooth muscle contractility in the ileum and colon. Regular coffee (Coff, blue) and decaffeinated coffee (Dec, pink) (250 mg in 2 mL water) was administered by oral gavage daily for 3 days. Sham rats were treated similarly, but with 2 mL of saline. Rats were euthanized 3 days later. Colon motility was measured by counting the time needed to expel the inserted pellet from the anus (A). The ileal (B) and colonic (C) muscle strips were isolated from the rats, and their longitudinal muscle contractility was recorded in muscle bath. The smooth muscle contractile response to acetylcholine (ACh) was determined. N = 6 or 5 rats in each group. * p < 0.05 vs. sham. Sham (Sh). Coff, regular coffee; Dec, decaffeinated coffee.