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In vitro effect of coffee on ileal muscle contractions. Ileal muscle strips were isolated from naïve rats, and their longitudinal muscle contractility was recorded in muscle bath (A). The in vitro effects of regular coffee (Coff) and decaffeinated coffee (Decaf) at different concentrations (0.1~10 mg/mL) on muscle contractility in the first 2 min after each dose was measured (B). N = 4 independent experiments in 4 rats.
