Table 2. Flow cytometry antibodies.
| Antigen | Fluorophore | Clone | Company | |
|---|---|---|---|---|
| Anti-mouse antibodies | CD19 | PE or FITC | ID3 | Thermo Fisher Scientific |
| CD11c | APC | N418 | BioLegend | |
| CD11c | Alexa Fluor 700 | N418 | Thermo Fisher Scientific | |
| T-bet | PerCpCy5.5 or PE | 4B10 | Thermo Fisher Scientific | |
| IgD | PE-Cy7 | 11-26c | Thermo Fisher Scientific | |
| IFNγ | APC | XMG1.2 | Thermo Fisher Scientific | |
| TNFα | PE-Cy7 | MP6-Xt22 | BioLegend | |
| IL-10 | Pacific Blue | JES5-16E3 | BioLegend | |
| IL-17A | PE-Dazzle594 | TCII-18H10.1 | BioLegend | |
| CTLA4 | PE-Dazzle594 | UC10-4B9 | BioLegend | |
| PDL1 | SB436 | MIH5 | Thermo Fisher Scientific | |
| PD1 | SB436 | J43 | Thermo Fisher Scientific | |
| IDO | PerCpeFluor 710 | mIDO-48 | Thermo Fisher Scientific | |
| Fas | FITC | SA367H8 | BioLegend | |
| CD27 | PE-Cy7 | LG.7F9 | BioLegend | |
| CD45 | PerCpCy5.5 | 30-F11 | Thermo Fisher Scientific | |
| CD4 | Alexa Fluor 700 | RM4-5 | Thermo Fisher Scientific | |
| CD8 | APC-eFluor 780 | 53-6.7 | Thermo Fisher Scientific | |
| FoxP3 | FITC | FJK-16S | Thermo Fisher Scientific | |
| CD3e | eFluor 450 | 500A2 | Thermo Fisher Scientific | |
| IL-6 | eFluor 450 | MP5-20F3 | Thermo Fisher Scientific | |
| GM-CSF | PE-Cy7 | MP1-22E9 | Thermo Fisher Scientific | |
| BAFF | Biotin | IC9 | Enzo | |
| Anti-human antibodies | CD19 | APC-eFluor 780 | HIB19 | Thermo Fisher Scientific |
| IgD | FITC | IA6-2 | BD Biosciences | |
| CD11c | Alexa Fluor 700 | B-ly6 | BD Biosciences | |
| CD21 | PerCpCy5.5 | Bu32 | BioLegend | |
| CD20 | Pacific Blue | 2H7 | BioLegend | |
| CD27 | PE-Cy7 | LG.3A1 | BioLegend | |
| Fas | PE Texas Red | DX2 | BD Biosciences | |
| PD1 | PE | MIH4 | Thermo Fisher Scientific |