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. 2022 Nov 25;8(47):eabq4882. doi: 10.1126/sciadv.abq4882

Fig. 2. Effects of hypothermia on tumor cell cycle, metabolism, and cytokine synthesis.

Fig. 2.

(A) Percentage of cells in each stage of the cell cycle after 3 days of 37°C, 3 days of 25°C, or 7 days of 25°C (n = 3). (B) Amount of intracellular ATP from the tumor cell lines when grown for 3 days at 37°C, 3 days at 25°C, or 7 days at 25°C (n = 3). Specific adjusted P values are provided in table S4. (C) Metabolite production or consumption per cell surface area across cell lines and temperatures after either 3 days of 37°C or 3 days of hypothermia (30°, 25°, and 20°C) (n = 3). (D) Concentration of cytokines (IL-6 and IL-8) collected in the medium at different time points and temperatures (n = 3). All studies used two-way ANOVAs with post hoc Dunnett’s multiple comparisons test (*P < 0.05, **P < 0.01, ***P < 0.001, and #P < 0.0001). All graphs show means ± SD.